Association-dissociation behavior of sesame alpha-globulin in electrolyte solutions.
نویسندگان
چکیده
The major protein fraction, alpha-globulin, of sesame seed (Sesamum indicum L.) contains subunits which are associated predominantly by hydrophobic interactions. Effects of various salts show the following effectiveness of anions in dissociating the proteins, SO4(2-) less than Cl- less than Br- less than ClO4- less than SCN- less than or equal to I- less than CCl3COO-, the first two members being association-inducing ions. CCl3COONa is found to be the most effective among the series in causing dissociation. The cations Li+, Na+, K+, and Cs+ induce association, the order of effectiveness being Cs+ approximately K+ greater than or equal to Na+ greater than Li+. The low concentration of salts (anions) necessary to induce dissociation does not involve a detectable change in protein conformation. The discrepancy between the effectiveness of the anions in dissociating the protein and the Hofmeister pattern of these ions has been discussed.
منابع مشابه
Association-Dissociation Behavior of Sesame a-Globulin in Electrolyte Solutions
The major protein fraction, a-globulin, of sesame seed CS’esamum indicum L.) contains subunits which are associated predominantly by hydrophobic interactions. Effects of various salts show the following effectiveness of anions in dissociating the proteins, SO,*< Cl< Br< C104< SCN4 I< CCl,COO-, the first two members being association-inducing ions. CC1,COONa is found to be the most effective amo...
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 252 1 شماره
صفحات -
تاریخ انتشار 1977